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Heterozygous MTHFR Gene Mutation | Allergy Research Group

Calcium Imaging: To visualize the rapid intracellular calcium mobilization triggered by GHSR-1a, cells can be pre-loaded with a fluorescent calcium indicator dye like Fura-2 AM or Fluo-4 AM

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Ser and Ala are typical, conservative replacements for Cys when mutagenesis studies are conducted, as the goal is ordinarily to remove the thiol functionality but introduce only minimal perturbations in structural integrity in the proteins that are mutated